Insertion junction: CLIP2.008858_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.008858
Chromosome:chromosome 11
Location:2218055
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467721 (1 of 1) IPR027446 - Variant surface glycoprotein C-terminal domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCTTAGTAACATCTGACATGTCATCCTATCGTTCCGTTGGTCAACGT
Internal bar code:TGTCAGAAACGAAATCAGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:5093
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:86
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTATGTGAAGGGGTGTGC
Suggested primer 2:CTGTGCTGTTGTGCAATGCT