| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.008887 |
| Chromosome: | chromosome 10 |
| Location: | 746500 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422650 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGCGGCCGGAACGTGCCACACAGGCGAACACCACACAGTTGGTTGC |
| Internal bar code: | GAATGATATTGTCTGATGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2363 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCACACGAGCTCTTGGAC |
| Suggested primer 2: | TGGAAGCAATGCAGCACAAC |