Insertion junction: CLIP2.008889_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.008889
Chromosome:chromosome 1
Location:2340056
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTCTGTGTTTGATTGCTCTGCGGGACCCAACCCCAGTGCCTTCTTTG
Internal bar code:CTAGTGCTGTGGGGCTCTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2841
LEAP-Seq percent confirming:95.9184
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTGGACACGTTCGATT
Suggested primer 2:CGTGTGTAGGGTGAGTGCTT