| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008910 |
| Chromosome: | chromosome 7 |
| Location: | 5121377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348250 | (1 of 1) K14840 - nucleolar protein 53 (NOP53, GLTSCR2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGGCGAGGAGGAGGAGGGCGGCGAGGGCGGCGCGGCGGCGGCGGCG |
| Internal bar code: | GGGTTGGCTAATGCTATTACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCACAGAAAGACGCAC |
| Suggested primer 2: | GAGGGAGGGTTGTCGTCAAG |