| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008912 |
| Chromosome: | chromosome 6 |
| Location: | 4768998 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279700 | (1 of 1) PTHR15535:SF22 - PROTEIN RLIB | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGACTCAGACGGCGGCCCCGGGCACTCGTTGGGTGACTGTGCGCGAC |
| Internal bar code: | AATGTATTTGCTGATACATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4912 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACCTTGCCCTCGATTCA |
| Suggested primer 2: | GTTTTGGTCACGCCTCACAC |