| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.008932 |
| Chromosome: | chromosome 10 |
| Location: | 3383551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443801 | (1 of 3) PF13401 - AAA domain (AAA_22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTTCTCCGCCGCAGCTCAGCCCCTCCGCCCAAACCCCAACCCTGCTC |
| Internal bar code: | TATAGCGCGGTTGGCGACTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 10.3448 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGCAGGGGAAATCAGT |
| Suggested primer 2: | CTCGTAGATCGTTGGTCCGG |