Insertion junction: CLIP2.008979_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.008979
Chromosome:chromosome 13
Location:312240
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801511 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGTGCCCACACACATACGCACGCACCTGGCTGGCGACGTTGAAGTAG
Internal bar code:GAAGGAAGGTAATATCTCATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3299
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGGTATCGTACAAGCTCC
Suggested primer 2:CAGAAGAGGCCGACTGAGTG