| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.008997 |
| Chromosome: | chromosome 1 |
| Location: | 7169569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051350 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGTAGCGCACAGTGAACGGTGACAGGTAGCCCTGCATTTATGAGGAT |
| Internal bar code: | TTCAACTTGTTATTTCGCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2093 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGCATGGGATACGGCA |
| Suggested primer 2: | ATTGGTACGCTCCATAGCCG |