| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.008999 |
| Chromosome: | chromosome 2 |
| Location: | 4327458 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105026 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCTCCGCCGCCAACGTGATCCGTGTGCTCCTCCTGCTGAAGCTGATG |
| Internal bar code: | GAACCCGCATCTGACGACTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCTTCTGTGTGCCAGCGA |
| Suggested primer 2: | TACTGGCACAAATCGCGACT |