Insertion junction: CLIP2.009011_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009011
Chromosome:chromosome 3
Location:6307677
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192100 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGGTCATCGTGACGGGTGCGTGCGCGCGGGACCTGTATGTGTGTGTG
Internal bar code:TGGTTGCGAGGGGGTCAAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1769
LEAP-Seq percent confirming:93.617
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAACACAGTGGGCAAAGG
Suggested primer 2:TTCCTAGCACGCAGAAGCAA