Insertion junction: CLIP2.009015_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009015
Chromosome:chromosome 7
Location:446875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315350 (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTACTGCCGGCGGCTGCCCAAGGGCCGCGGCGGCTTCGGCAGCGCTGA
Internal bar code:CCGGGCAGTGGATTCGAGTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:392
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAGAAGGAGGTGGACGC
Suggested primer 2:GCGCTTGCTCGATTAGTGTG