Insertion junction: CLIP2.009016_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009016
Chromosome:chromosome 2
Location:911058
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g079000 POB21 Proteome of basal body 21 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTAGGGGAGGCAGCGGGTGGGAGGGGTTAGGAAGGTCAGAGAACGTGT
Internal bar code:GTTTATCATTACGGACATTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:981
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGGTGTGTGGCTGATGAC
Suggested primer 2:GCGTTGATGTCTGCGCTTAG