Insertion junction: CLIP2.009018_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009018
Chromosome:chromosome 3
Location:493285
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145027 (1 of 14) PF13371 - Tetratricopeptide repeat (TPR_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCATCGCATACACTGTCTTTGCGCAACGTTTTCCTTGTGCTCTTTAA
Internal bar code:AGGGAGCGGGACAAATACGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2779
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCTAATCAGTCCCTGAC
Suggested primer 2:GCACGCGTGTATTTCGAGTC