| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009023 |
| Chromosome: | chromosome 1 |
| Location: | 504941 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003000 | outside_mRNA | ||
| Cre01.g003050 | SEC8 | (1 of 1) PTHR14146:SF0 - EXOCYST COMPLEX COMPONENT 4; Component of the Exocyst Complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTATTGACCAGCGCCTCCTGCCGGTCCCTGAAGAGGGCGAACCTTGGC |
| Internal bar code: | AGCGCTTCCGATTGAACCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1371 |
| LEAP-Seq percent confirming: | 71.7949 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGCTGAAGGCCAAGG |
| Suggested primer 2: | CGGTATTTGTGAGCCTGGGT |