Insertion junction: CLIP2.009039_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009039
Chromosome:chromosome 6
Location:409439
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g251650 PTC1 (1 of 1) K14430 - phosphate transporter (PHO87_91); Low-affinity phosphate transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCAGCCAGCGCGCGTGCGTGCGCAGCCACCTGCGGCGGCAGCGGCGTG
Internal bar code:CGCACGTGCAGGTTTTCGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1077
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGACAGGGTCGATACTGA
Suggested primer 2:CAAGTGTGTGTGTGCGTGAG