Insertion junction: CLIP2.009042_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009042
Chromosome:chromosome 1
Location:7167573
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g051350 (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCAGCGGCTTGGCCGTGAGTCGCAGCGGTCTCAGAACAAGCGGACAGG
Internal bar code:CTGGTTACCCTTTTGCGAATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1969
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTACTGATGGGAGCTCGA
Suggested primer 2:CGGCATGTCTGTGTTTCTGC