Insertion junction: CLIP2.009052_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009052
Chromosome:chromosome 17
Location:4769327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g733150 HKR7,HKR78,HKR8,COP12HKR6,COP,COP11 Histidine kinase rhodopsin 7/8; (1 of 1) PF00072//PF00512//PF01036//PF02518 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGGAGGATTGCGGCTGCGTGGGGGCTGCTGCGGTGACTGTTTGGGTC
Internal bar code:CATGACTTTTAGTGGTACGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2152
LEAP-Seq percent confirming:98.5507
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGTAGGATGGGGTGGTC
Suggested primer 2:CTTGGTCGGACTGTCCCTTC