| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.009056 |
| Chromosome: | chromosome 2 |
| Location: | 5572394 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115350 | (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACGCCCGTGGAAGACCACTGGCGGCCCCGGGCGCGGCGGCGGTGTC |
| Internal bar code: | TAAGTATAGCTTGTTAAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1418 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGAAACGGTGTGGTGATA |
| Suggested primer 2: | CTTGACCCAGCCTACCACTG |