| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009062 |
| Chromosome: | chromosome 5 |
| Location: | 1499057 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234655 | (1 of 1) K17550 - protein phosphatase 1 regulatory subunit 7 (PPP1R7, SDS22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCGAGTCCTTTTCTTATTACCTATGGGGCAGGGCACTACCACTAAT |
| Internal bar code: | CTTAGGTCCGGGCGGCTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGTGGAGATGCCGTTGTG |
| Suggested primer 2: | TGGCTAGCAACAAGGTGAGG |