| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.009066 |
| Chromosome: | chromosome 16 |
| Location: | 3642484 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675602 | (1 of 1) K12669 - oligosaccharyltransferase complex subunit gamma (OST3, OST6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATTGTCCTTCCATTACCACAGAATGTTGCAGGGCTGTCTGCCCTGCG |
| Internal bar code: | CCCCTGCGTCCAACCTCTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3340 |
| LEAP-Seq percent confirming: | 97.076 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 171 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACGGGTGTAGACTCAA |
| Suggested primer 2: | GGGACTTGATGTGCATGGGA |