Insertion junction: CLIP2.009072_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009072
Chromosome:chromosome 10
Location:1075185
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g425251 (1 of 5) IPR008274 - Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTCGCCGCTAATCGCGCTTTGGCGGCTGCGCAGGGCGTGCACTGCTGC
Internal bar code:CTGTAATGGTGTTTCGGGTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2704
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGAAGGCTCAGCTTGGAC
Suggested primer 2:CATCGCCGTTAACTGCATCG