Insertion junction: CLIP2.009082_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.009082
Chromosome:chromosome 3
Location:706732
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145847 (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTACTGCCGCTGCAACGCGGCAGCAGCAGTCAGCGGGAGTCCCCGGC
Internal bar code:TATGCGTGTTAGAACTGTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2369
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCTCGCAGTTCACACTCA
Suggested primer 2:GACCCAGATCCCACTTGCTC