Insertion junction: CLIP2.009117_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009117
Chromosome:chromosome 16
Location:308744
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g694204 (1 of 1) K15177 - RNA polymerase-associated protein LEO1 (LEO1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCAGGGCGGTCAAAGCGGTCCAGGGGCATACGGAATGTGGTGCAGAG
Internal bar code:ACATCTCTTACGGTATTGCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:981
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTGCTCACAAACTGCTA
Suggested primer 2:CCAAACAACGACAGCAGGTG