Insertion junction: CLIP2.009146_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009146
Chromosome:chromosome 2
Location:2721726
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGTCTGGAGACGACCCCAGAAGTCAGCTACGACGGCAAGTCCAATGCT
Internal bar code:TTGAAGGCTGGTTAGAACTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3658
LEAP-Seq percent confirming:96.8553
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:159

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCTTGCAAGGGTTACTA
Suggested primer 2:TAAGCGGGGTATCTGGGTCA