| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.009155 |
| Chromosome: | chromosome 7 |
| Location: | 122463 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312950 | HEL32 | DEAD/DEAH box DNA/RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTACCCTCTGTCCAGTGCAGCAGCCGTCTCACTTTGTGTCGCTGCATT |
| Internal bar code: | ATCGTTCAGTGGTGCTCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3290 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGGGACTACTAGCTCGC |
| Suggested primer 2: | GCAAGCCGAAGAGCAATAGC |