Insertion junction: CLIP2.009171_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009171
Chromosome:chromosome 4
Location:1977937
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g218900 (1 of 2) PTHR10887//PTHR10887:SF333//PTHR13779//PTHR13779:SF20 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED // HOLLIDAY JUNCTION DNA HELICASE RUVB-RELATED // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACCCCAAAGCGATCTTTGACGATCTTATCGGCTGCAGGTGAGTCGTG
Internal bar code:TTTTACACTCTCTTCCAAAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3240
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:GATAAAGTGCAGCGCAGTCG
Suggested primer 2:GGGCTATGAGGACCAGATGC