Insertion junction: CLIP2.009180_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009180
Chromosome:chromosome 6
Location:5780541
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g288908 (1 of 30) PTHR24349//PTHR24349:SF79 - SERINE/THREONINE-PROTEIN KINASE // NITROGEN NETWORK KINASE 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCATGTGCACGCTGCCACACCTCCACAGCTGCAGCGCACACGTCAAC
Internal bar code:GGTTTCAACTTAAGATTAGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4122
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAGTAGCTCTGTCCGCTG
Suggested primer 2:TGATACAGATGTACGCCGCC