| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.009185 |
| Chromosome: | chromosome 13 |
| Location: | 1534000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572800 | TILS1,TILS | tRNA-Ile Lysidine synthase, organelle; (1 of 1) 6.3.4.19 - tRNA(Ile)-lysidine synthetase / Isoleucine-specific transfer ribonucleate lysidine synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCGCCGCGGTCGCGGCGCCCCCTGAACAGGAACGCAAGTTGACGAGG |
| Internal bar code: | CATTCAGAACTCAACCAGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1157 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCTCACATCGTCCCCA |
| Suggested primer 2: | CATGTTGTTGAAAGGGCCCG |