| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.009244 |
| Chromosome: | chromosome 12 |
| Location: | 2643860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505750 | RPB9 | DNA-directed RNA polymerase II, 14.5 kDa polypeptide; (1 of 1) K03017 - DNA-directed RNA polymerase II subunit RPB9 (RPB9, POLR2I) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGCCCGCGCTTGCGAGACGCCATGGCCGCAGTGCGATGCCGCCGCGC |
| Internal bar code: | TCGCCGAGCTGTACCGTCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3082 |
| LEAP-Seq percent confirming: | 84.8485 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGTGTGCAGGAAGTGTG |
| Suggested primer 2: | GCATGGCGTAACCCGAAATC |