| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009245 |
| Chromosome: | chromosome 11 |
| Location: | 1766316 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467950 | FAP383 | Flagellar Associated Protein 383 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCGGCCGGACGTGCTAACGCCCGCGTCGCCGCCGCCGCCGCCGCCTG |
| Internal bar code: | CTATACAGAGCGTGCTCTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 10.8108 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCAAACACGCACAAAAC |
| Suggested primer 2: | CACACACACCTCCTCACCTC |