Insertion junction: CLIP2.009252_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009252
Chromosome:chromosome 2
Location:6967677
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389319 (1 of 2) PTHR11266:SF17 - PROTEIN MPV17 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAACGCCTATGAGCGCTCCCTGCGCCGGCACCCAGTGCTGACGCAGGC
Internal bar code:TATTGGCCGGCCGCGCGCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1297
LEAP-Seq percent confirming:9.52381
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:76
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:GGACAGGAAGCAGACGTCAA
Suggested primer 2:TTCCGCGCCACAAAATTCTG