Insertion junction: CLIP2.009260_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009260
Chromosome:chromosome 7
Location:4629044
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344500 GT90-10,GT90F10 (1 of 2) K13667 - protein glucosyltransferase (RUMI, KTELC1); GT90 family protein 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTACTCGTTTACCCCTGCACAGTGCGTCAACAAGGCTCCACCAGTCATG
Internal bar code:TGAATGGCCTGTCACCGTGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1882
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATGTCCTTCTCCGCTTT
Suggested primer 2:TTGCAAGGTCCAGCTACCAG