Insertion junction: CLIP2.009281_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009281
Chromosome:chromosome 12
Location:9336735
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g541904 (1 of 1) PF08755//PF13369 - Hemimethylated DNA-binding protein YccV like (YccV-like) // Transglutaminase-like superfamily (Transglut_core2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCTGGCGGGCACGGGAGTGGCTGTGGCCCGGCACCACGTGCGGCCGC
Internal bar code:ACCTTCTTATAACTCTTCTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACATATCACAAACGCGTGCG
Suggested primer 2:CGGTGTCCTTACCACATGCT