| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009300 |
| Chromosome: | chromosome 1 |
| Location: | 1632536 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008850 | HLM2 | (1 of 1) PF12931//PF12932 - Sec23-binding domain of Sec16 (Sec16_C) // Vesicle coat trafficking protein Sec16 mid-region (Sec16); Histone-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGTGGGCGGGAAGGCTGTGTGCGACTGGTGGTCCTCACCCTCGTCA |
| Internal bar code: | GATTATGGAGCTGTGGGACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2369 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTTGACGACATGGGCGA |
| Suggested primer 2: | AGGTCGTGTAAGAAGCACCG |