Insertion junction: CLIP2.009300_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009300
Chromosome:chromosome 1
Location:1632538
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g008850 HLM2 (1 of 1) PF12931//PF12932 - Sec23-binding domain of Sec16 (Sec16_C) // Vesicle coat trafficking protein Sec16 mid-region (Sec16); Histone-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGGGCCGCCGCGAACGGCTGACGAGGCGGCGCGCGCGCGCATCAGTC
Internal bar code:GAGGGATCCGGAGGGAAACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1320
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCGTGTAAGAAGCACCG
Suggested primer 2:TTCTTTGACGACATGGGCGA