Insertion junction: CLIP2.009330_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009330
Chromosome:chromosome 12
Location:7510443
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g556650 APC4,DIV77 (1 of 1) K03351 - anaphase-promoting complex subunit 4 (APC4); Anaphase promoting complex subunit 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTAGGGGGGCGGGAGAGGGAGAGGAAGGGGATCCAAAAGGGGTGTTGCT
Internal bar code:TGGACGGTGATGTCCGATTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:230
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCAGTGAGATAGAGCAGG
Suggested primer 2:TAAGGGGAGAGGGAGGGTTG