Insertion junction: CLIP2.009331_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009331
Chromosome:chromosome 12
Location:9580040
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g539750 (1 of 1) PF09649 - Histone chaperone domain CHZ (CHZ) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGGGTCAAAGGGCGGCCGAAAGAAGAAGGGTGGGCGGGGCTCGGATG
Internal bar code:ACTCTCGGTAACTTTGAATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:65.2174
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGGCAAAACAGAGAGCA
Suggested primer 2:CGCCCGCTTTACAACTATGC