Insertion junction: CLIP2.009335_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009335
Chromosome:chromosome 7
Location:569177
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g316500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCCCCTGATTCCGGAAGTCGCCGCAGCAGCACACACACACACACAC
Internal bar code:TTGACGAATGACGCACATACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1037
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGTCAGCTTTGCGTTCG
Suggested primer 2:GAAAAACGACACCACACGGG