Insertion junction: CLIP2.009346_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.009346
Chromosome:chromosome 2
Location:2758549
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGGCCGCAGCGAGCTGCTGGGGGCTCTGCGCACCGAGGTGGTGGCGC
Internal bar code:ACATCTATCTTGCTCGGTCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1500
LEAP-Seq percent confirming:9.30233
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTCTGATACTGTTTGCG
Suggested primer 2:AGGGATTACCGGAAGAGGCT