Insertion junction: CLIP2.009350_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009350
Chromosome:chromosome 8
Location:1840312
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g367150 MFT23 Major facilitator superfamily transporter; (1 of 22) IPR011701//IPR020846 - Major facilitator superfamily // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGACCCGTACGCGAGCATGCGGACGGTGCGCGTGAGGAACAGCACCGC
Internal bar code:GTAATGACGTCGATCCATTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1365
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTCGCTTTTGTGTGCAA
Suggested primer 2:CTATCCCAATGCGCATTGCC