| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009354 |
| Chromosome: | chromosome 16 |
| Location: | 613934 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692050 | SYP3 | (1 of 1) K08490 - syntaxin 5 (STX5); ER-Golgi Qa-SNARE protein, Syntaxin5/Syx5/Sed5/Syp3-family (Qa.II) | CDS |
| lncRNA_TCONS_00196237 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGAGGCGGCGAGGAAGCTGGGCGTCGGCGCGCTGGGTGCTGAGGAGC |
| Internal bar code: | CACGGTACCAAAAAGGATCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2299 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAGACGCTGGAACTAGT |
| Suggested primer 2: | ACTCGGGCATTGAAGACTGG |