| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.009359 |
| Chromosome: | chromosome 7 |
| Location: | 6295345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356800 | OPR33 | OctotricoPeptide Repeat protein 33; (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCTGTAGCTGCAGCAGCTGCTCCAGCTCGTCCAGGCACGTGACGGA |
| Internal bar code: | TAAATCAGGTAGAATCTTTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2213 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTCTGACCTGTACGG |
| Suggested primer 2: | CATCCACAAGCTCCAGACCC |