Insertion junction: CLIP2.009363_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.009363
Chromosome:chromosome 13
Location:3850330
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAAGCGATGACTTACGCGGGAGATAAACGTACATAGGAGGAAAGGTGG
Internal bar code:AGTTACTGATAGACATTGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1953
LEAP-Seq percent confirming:94.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CTACATGCAAACGAGCGCTC
Suggested primer 2:GGAGAGGACGTAGGTCACCT