| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009368 |
| Chromosome: | chromosome 10 |
| Location: | 1014109 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424900 | PHX13,PFH1 | Prolyl 4-hydroxylase 1; (1 of 2) PTHR10869//PTHR10869:SF66 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // OXIDOREDUCTASE, 2OG-FE(II) OXYGENASE FAMILY PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAACACGGTCTCCCCGCCCTCCAGCACCTCGCTGAGGTACAGCAGCAC |
| Internal bar code: | TTGGCAGTCGGAGTAATATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1454 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTTGTGTCAGCATGGG |
| Suggested primer 2: | ACTTCTCGCCCTTCTTGACG |