Insertion junction: CLIP2.009404_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009404
Chromosome:chromosome 3
Location:732104
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145967 (1 of 2) K15437 - aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1, ARC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGAGGCGACAGCGTCCGGGCAATGTTCTTTCATCAATGGCCGTGAGTC
Internal bar code:CCTGGCCTTCCATCCATCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:271
LEAP-Seq percent confirming:80.9524
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACAGGATCGTGTGGTGG
Suggested primer 2:GGTATTTACAACCCCCGCCA