| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009407 |
| Chromosome: | chromosome 9 |
| Location: | 2949714 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390200 | IPY2 | Inorganic pyrophosphatase; (1 of 4) K01507 - inorganic pyrophosphatase (ppa) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGACTCATCATCATCACGCACCTTGTAGGTACGGAAGAACTCGTACAG |
| Internal bar code: | GGTTGGATGTATAATAAGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2181 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTCACTGCTGGAGGAA |
| Suggested primer 2: | CTCACACCACCACTACCACC |