| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009409 |
| Chromosome: | chromosome 1 |
| Location: | 8130940 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g066552 | TRXf1,TRXF1 | Thioredoxin f1, chloroplastic; (1 of 7) 3.5.1.52 - Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase / N-oligosaccharide glycopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTCAATCTGCTTGCTGTTGCGGTAGAGGTGGAAGGTGGGCAGCGCC |
| Internal bar code: | TCTTCCGTTTTGCAGACTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2347 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAACCTGTCTCCTGCAT |
| Suggested primer 2: | ATGCCCCAATTCTTCCCCTG |