| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.009417 |
| Chromosome: | chromosome 2 |
| Location: | 1705612 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085950 | (1 of 1) PTHR33415//PTHR33415:SF3 - FAMILY NOT NAMED // EMB514 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCCTTGCGGCCGCCGGAGCGTGCGCACATGCACGCAAGGCCTGCTG |
| Internal bar code: | TTTCGGATGCGCGAGCTTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2118 |
| LEAP-Seq percent confirming: | 92.4528 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCTGCACATAATCCAAG |
| Suggested primer 2: | AAGCTGTGGAAGAAGGAGCC |