| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.009426 |
| Chromosome: | chromosome 3 |
| Location: | 5793992 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187700 | HEL19 | (1 of 1) PTHR24031//PTHR24031:SF252 - RNA HELICASE // SUBFAMILY NOT NAMED; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATTTCCCCTCGCCCCCACCTCCATGTGGTAACGACTTCGTCTGCAGT |
| Internal bar code: | TGATTATAAGCGCTTTGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGAGAGGGGAGAGGTT |
| Suggested primer 2: | CCCGGTCAGCATTCTCCTTT |