Insertion junction: CLIP2.009447_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.009447
Chromosome:chromosome 7
Location:5754790
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353200 (1 of 1) IPR006073//IPR016496//IPR025121//IPR027417//IPR030394 - GTP binding domain // GTPase HflX // GTPase HflX, N-terminal // P-loop containing nucleoside triphosphate hydrolase // HflX-type guanine nucleotide-binding (G) domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGGAGGGGTGCGGGGCTGGGTCAGGGTGCGGGTGCGCAGCTTGGCGC
Internal bar code:GTAAACGTCCAAGGTGACCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3557
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCACTCTCCCCGGTCCTA
Suggested primer 2:ATCACAGACCGAAGCCAAGG